Experiment 2: Protein Experiment ~ Biochemistry

 

biochemistry lab report

Biochemistry Lab Report - Free download as PDF File .pdf), Text File .txt) or read online for free. Lab Report for my Biochemistry course at DePaul University3/5(2). Apr 02,  · Biochemistry. Tuesday, 2 April Experiment 2: Protein Experiment Introduction: There is no single protein assay method that yields absolutely accurate results. The only true and accurate method for determining protein concentration is by acid hydrolyzing a portion of the sample and then carries out amino acid analysis on the. The Biochemistry Formal Laboratory Report Title Page: (5 points) Descriptive title Course Name, Number, and Section Date Experiment Completed Name of Person Submitting Report (Underlined) Name of Lab Partner(s) Introduction: (20 points) The introduction should state the purpose of the experiment and should contain a brief.


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Quite interesting and nice topic chosen for the post Nice Post keep it up. Excellent post. I want to thank you for this informative post. I really appreciate sharing this great post. Keep up your work. Thank you for the post. I was able to direct myself how to write a discussion. Experiment 2: Protein Experiment. To determine protein concentration in various type of protein content.

To determine protein concentration using two types protein assays that are Biuret assay and Lowry assay. In the experiment to determine the protein concentration, Biuret assay and Lowry assay is used.

The test is so named because it also gives a positive reaction to the peptide-like bonds in the biuret molecule. Standard Protein. Absorbance nm.

Samples Protein. Types of sample egg. Absorbance based on graph nm, biochemistry lab report. Chicken 1 Normal Chicken. Chicken 2 Ayam Kampung. Omega 3, biochemistry lab report.

Quail Puyuh. Graph and Calculation. Full view. Larger view. A Biuret assays. Dilution of the solution samples:. The actual concentration of the protein in the samples:. The subunits which make up proteins are amino acids.

The amino acids biochemistry lab report joined together by dehydration synthesis to forms chains, which are hundreds of amino acids long which is called proteins. Proteins function as enzymes or as structural units in cells. They do most of the work in a cell.

Almost all of the exciting stuff such as metabolism, memory, hormone action, and movement involves proteins. In this lab, we have learnt method of measuring protein concentration, biuret assay. The biuret reaction is a method that can be used to determine the amount of soluble protein in a solution.

The biuret reagent copper sulfate in a strong base reacts with peptide bonds which join amino acids to form proteins and changes colour when it does so. The spectrophotometer has been used to measure the intensity of the colour produced. The more protein present the darker the colour. In order to quantitatively determine how much protein is represented by a particular absorbance reading it is necessary to construct a standard curve. This graph is called the standard curve for assay, and can be used to convert the absorbance readings for the experimental samples eggs from duck, chicken 1 ayam biasabiochemistry lab report, chicken 2 ayam kampungbiochemistry lab report, omega 3, quail into a protein amount or concentration.

From biochemistry lab report graph we can see that, the highest concentration of protein is chicken 1 and the lowest is omega 3.

But for the real result actually the omega 3 will get the highest and the chicken1 will get the lowest. So, from the experiment maybe there are some mistake happen while conducted it. Error that occurred could biochemistry lab report the mixing of using the same pipette. Lowry method is one of the ways to determine the protein concentration in a solution. However it needs two-step procedure which requires a minimum of 40 minutes incubation time.

The sensitivity of Lowry Assay reagent is greatly enhanced over that of the Biuret total protein reagent. The reading of colour at nm for Lowry Assay is recommended because at this wavelength, few other substances absorb light.

The amount of light absorbed at nm is directly proportional to the amount of protein in the sample. Throughout this lab, we can conclude that there are two ways of measuring protein concentration in a solution.

It is either by using biuret biochemistry lab report or Lowry assay. Both can be used to measure the protein concentration in a solution but the most accurate way to measure the concentration is by using Lowry assay.

From the graph plotted, chicken 1 contain the most protein while omega 3 contain the least. The results supposed to be vice versa, biochemistry lab report, omega 3 should contain the most protein whereas chicken 1 the least. This happened due to some mistakes we make during pipetting the samples. Jhon mac 15 February at Unknown 3 October at Unknown 4 October at Unknown 15 February at Unknown 21 October at Unknown 11 January at Unknown 5 March at Newer Post Older Post Home, biochemistry lab report.

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Experiment 3: Experiment on enzyme ~ Biochemistry

 

biochemistry lab report

 

Biochemistry Lab Report - Free download as PDF File .pdf), Text File .txt) or read online for free. Lab Report for my Biochemistry course at DePaul University3/5(2). Apr 02,  · Biochemistry. Tuesday, 2 April Experiment 2: Protein Experiment Introduction: There is no single protein assay method that yields absolutely accurate results. The only true and accurate method for determining protein concentration is by acid hydrolyzing a portion of the sample and then carries out amino acid analysis on the. The Biochemistry Formal Laboratory Report Title Page: (5 points) Descriptive title Course Name, Number, and Section Date Experiment Completed Name of Person Submitting Report (Underlined) Name of Lab Partner(s) Introduction: (20 points) The introduction should state the purpose of the experiment and should contain a brief.